Our current research focuses on the neurobiology of sleep and sleep-related breathing in the animal models \\\"in vivo\\\" of healthy aging and neuropathology of the most common neurodegenerative diseases of elderly (such as the Alzheimer’s Disease (AD), Parkinson’s Disease (PD) and different types of dementia), aimed to give a new insight into understanding of normal and abnormal sleep, the mechanisms of the sleep related behavioral disorders, particularly sleep apnea syndrome, and to identify the neural networks responsible for modifying the regulation of sleep and breathing pattern in different sleep/wake states.
Since underlying pathology of AD and PD involves the selective loss of specific neuronal population, an important role of the cholinergic system in their etiology, and the ascending pattern of neurodegeneration progression from REM sleep-related brainstem regulatory structures to basal areas of the brain, in our research we use the rat models of unilateral or bilateral lesion of the nucleus basalis (NB; the main cortical cholinergic nucleus), and nucleus pedunculopontine tegmentum, (PPT; the main cholinergic nucleus of the brainstem - pons), performed by stereotaxically guided selective microinfusion of the excitotoxin in a nanoliter volume.
We follow the severity and progression of disorder caused by neurodegenerative process by electroencephalography (EEG): by the changes in EEG rhythms or certain EEG oscillations and by the sleep/wake states and NREM/REM cycling analysis. We correlate all changes within EEG with the changes in breathing pattern using the methods of linear and nonlinear analysis to estimate the onset, progression, and severity of the disorder. We believe that EEG potentially presents a rich source of still unrevealed information related to functional, physiological, and pathological status of the brain. Developing the new methods for EEG processing and modelling will significantly contribute in understanding the mechanisms of sleep regulation, detection of disease related sleep and sleep-related breathing disorders, and in estimation of the neurodegenerative disease progression. Complementary studies in humans and the animal models of human neurodegenerative pathology, will be highly enriched with the development of practical, sensitive and reliable methodology for early detection of the sleep behavioural disordered patients, who are at risk for developing Alzheimer’s and Parkinson’s Disease, or for prevention and alteration of the approaches for therapy.
From: 01.01.2011. To: 31.12.2014.
►Medical Center "Bežanijska Kosa", Belgrade
►Institute for Biological Research "Siniša Stanković", University of Belgrade
►School of Medicine, University of Belgrade
|Dr Jasna Šaponjić, senior research fellow||Institute for Biological Research "Siniša Stanković", University of Belgrade|
|Dr Aleksandar Kalauzi, research professor||IMSI, University of Belgrade|
|Dr Miodrag Vukčević||Medical Center "Bežanijska Kosa", Belgrade|
|Srdjan Kesić||Institute for Biological Research "Siniša Stanković", University of Belgrade|
|Jelena Petrović||Institute for Biological Research "Siniša Stanković", University of Belgrade|
|Jelena Ćirić||Institute for Biological Research "Siniša Stanković", University of Belgrade|
|Dr Vera Gardijan||School of Medicine, University of Belgrade|
|Dr Milica Obradović||School of Medicine, University of Belgrade|