University of Belgrade

Institute for
Multidisciplinary Research

Project information

Fishes as water quality indicators in open waters of Serbia

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Project ID OI173045
Project Title Fishes as water quality indicators in open waters of Serbia

Project Leader Dr Mirjana Lenhardt, research professor, IMSI, University of Belgrade
IMSI Status Manages the project

Financial Source Ministry of Education, Science and Technological Development of the Republic of Serbia


Category of Project Fundamental Research (OI)

 

PROJECT DESCRIPTION

Monitoring of the growing human impact on environment represents one of the research priorities in Europe. Recent studies show that less than 10% of all wastewater in Serbia is treated before being released into water bodies, which represents an actual threat for the quality of open waters in Serbia. Besides the influence of industrial and communal wastewater, open waters are also under the impact of runoff from arable land. Although it is not yet a member of EU, Serbia took measures which will contribute to fulfillment of basic goals of the EU Water Framework Directive (WFD), to which it is obliged as a signatory of the International Commission for the Protection of the Danube River (ICPDR), since this commission took responsibility for the implementation of WFD. As stated in the WFD, all qualitative parameters of aquatic ecosystem health, including biological parameters, should reach the satisfactory level by year 2015. In line with the WFD, fishes represent one of the most important elements of aquatic ecosystem biological quality, and should be used for the assessment of ecological status of rivers and other water bodies, where species composition, abundance, and age structure represent the minimal quantity of data that should be used in ecological status assessment. Furthermore, the condition factor and the data on fish population restoration and parasites are recommended by the WFD as supplementary parameters that should be analyzed.

The research within this project will include all parameters stipulated by WFD, as well as additional analyses of fish populations at different organizational levels that will be used in determination of negative anthropogenic impacts. Field and laboratory research will include several levels of biological organization: molecules, cells, tissues, organs, organisms, populations, and communities.

Molecular analyses will incorporate research of genotoxicity using the Comet Assay and micronucleus test. The Comet Assay is one of the most commonly used methods for collecting data on DNA damage. New additions to this method improved the sensitivity for identification of genotoxic agents and its easier application. The micronucleus test will be conducted as an additional genotoxicity assessment method. The sensitivity of both methods and their significance in evaluation of genotoxic effects on fish will be compared. Electroretinography will be applied at cell, tissue, and organ level, as well as histopathological analyses, analyses of heavy metal and micro-elements accumulation in particular organs, and determination of hepatosomatic (HSI) and gonadosomatic (GSI) indices. Electroretinography will be used as a particularly sensitive diagnostic procedure for identification of different intoxications and metabolic disorders in various fish species. The amount of heavy metal accumulation in natural fish populations, their distribution in different fish organs and tissues, as well as heavy metal concentration at different trophic levels of aquatic communities will be evaluated in order to determine the level of biomagnification through food chains. Histopathological alterations in different fish tissues will be examined in order to determine their possible correlation to heavy metal accumulation. All these analyses will allow the assessment of the potential of heavy metal bioaccumulation and histopathological alterations in fish organs as indicators of environmental pollution and its consequences.


PROJECT DURATION

 

From: 01.01.2011.   To: 31.12.2014.

 

PARTNERS

 

►Institute for Biological Research "Siniša Stanković", University of Belgrade

►Faculty of Biology, University of Belgrade

►Faculty of Sciences, Lucian Blaga University of Sibiu, Romania

►The Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia

 

PARTICIPANTS


No. Participant Institution
1.
Dr Mirjana Lenhardt, research professorIMSI, University of Belgrade
2.
Dr Miroslav Nikčević, senior research fellow IMSI, University of Belgrade
3.
Dr Aleksandar Hegediš, research professorIMSI, University of Belgrade
4.
Dr Branislav Mićković, senior research fellow IMSI, University of Belgrade
5.
Dr Zoran Gačić, research professorIMSI, University of Belgrade
6.
Dr Ivan Jarić, senior research fellow IMSI, University of Belgrade
7.
Dr Sonja Veljović Jovanović, research professorIMSI, University of Belgrade
8.
Dr Gorčin Cvijanović, research assistantIMSI, University of Belgrade
9.
Dr Filis Morina, senior research fellow IMSI, University of Belgrade
10.
Dr Marija Smederevac-Lalić, research fellow IMSI, University of Belgrade
11.
Dr Željka Višnjić-Jeftić, research fellow IMSI, University of Belgrade
12.
Dr Stefan Skorić, research fellow IMSI, University of Belgrade
13.
Dr Milica Jaćimović, research fellow IMSI, University of Belgrade
14.
Dr Marija Vidović, research fellow IMSI, University of Belgrade
15.
Dr Slađana Spasić, senior research fellow IMSI, University of Belgrade
16.
Uroš Ljubobratović, research traineeIMSI, University of Belgrade
17.
Dr Jasmina Krpo-Ćetković, assistant professorFaculty of Sciences, Lucian Blaga University of Sibiu, Romania
18.
Dr Angela Banaduc, associate professorFaculty of Sciences, Lucian Blaga University of Sibiu, Romania
19.
Dr Doru Banaduc, assistant professorFaculty of Sciences, Lucian Blaga University of Sibiu, Romania
20.
Vesna Đikanović MScInstitute for Biological Research "Siniša Stanković", University of Belgrade
21.
Dr Ilija DamjanovićThe Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia