University of Belgrade

Institute for
Multidisciplinary Research

Equipment

1. Department of Materials Science (MS)

2. Department of Life Sciences (LS)

3. Department of Natural Resources and Environmental Sciences (ES)

4. All Equipment

 

Department of Life Sciences (LS)

 

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Category Name / Description Image

Capital equipment

Spektophotometer Shimadzu 2501-PC (Shimadzu, Japan)

Apparatus is applied in spectrophotometric characterization of liquid samples and for conducting biochemical assays.
Temperature-controlled spectrophotometer; Wavelength range 200-800 nm

Capital equipment

Spectrofluorimeter and phosphorimeter FL3-221 P TCSPC (HORIBA JOBIN YVON)

Measurements of steady state and time resolved fluorescence and phosphorescence, on liquid and solid samples. There are two detectors in T configuration (photon counter and photomultiplier). For time resolved fluorescence diodes are purchased emitting at: 265, 278, 340, 370, 460, 488, 625 nm и 650 nm laser diode. Time resolved fluorescence can be measured in the range 100 picoseconds to 10 milliseconds.
Light sources: 450 W xenon lamp and microsecond UV xenon flash lamp.
Excitation range: 200-950 nm
Emission range: 200-950 nm
Possibility of temperature control in the measuring compartment in the range -20–80°C by using Peltier cooler.
Accessory for polarization fluorescence measurement.
Dewar vessel for keeping the sample in liquid nitrogen in the measuring compartment during measurement.
FluorEssence software.

Capital equipment

Heka-AM Systems-Axiovert Patch-Clamp System

The system is applied in elecrophysiological measurements. It contains microscope, amplifier, micro-manpuplator, electrode puller, interface.

Capital equipment

Gas Chromatograph GC-2010-Plus (Shimatzu, Japan)

High-sensitivity equipment for detection and quantification of volatile compounds in liquid and gaseous samples. Type of detection: MS; Carrier: He; Type of ionization: EI.

Capital equipment

Spectrofluorimeter FluoroLog 3 (Horiba, Japan)

Fluorolog-3 spectrofluorоmeter is used to measure and fluorescent and phosphorescent spectrum samples in liquid and solid form.
The main parts of the Fluorolog-3 spectrofluorometer system are:
State-of-the-art optical components (include double-grating monochromator in the excitation and emission path in an „L“ configuration range 200-800 nm, FL-1040 Phosphorimeter and F-3004 Peltier Smaple Cooler),
A personal computer,
DataMax for Windows, the driving softer.

Capital equipment

Electron paramagnetic resonance (EPR) spectrometer Varian E104-A (Varian, USA)

It is suitable both for liquid and solid samples, and can detect organic radicals, spin-trapped ROS, RNS, and other paramagnetic species. It can be used for detection of transition metals.
Apparatus operates in X-band, and it is equipped with cooling system with liquid nitrogen.
Cavity can be irradiated with light during the measurements.

Capital equipment

HPLC LC-20AB Prominence (Shimadzu, Japan)

In combination with adequate columns and mobile phases LC-20AB Prominence enables separation and characterization of wide spectrum of metabolites such as phenolic acids, flavonoids with anthocyanins, amino acids, carboxyl acids, ascorbate, glutathione and carotenoids.
Modular system consists of:
– degasser (DGU-20A3),
– binary pumps (LC-20 AD),
– auto-injector (SIL-20 AC),
– column oven (CTO-20A),
– diode array detector (SPD-M20A)
– fluorescence detector (RF-10-AXL).

Capital equipment

System for microwave digestion of samples under the high pressure.

Manufacturer: BERGHOF Instruments GmbH, Eningen, Germany.

Capital equipment

HPLC Waters LC-MS System (Milford, USA)

High-performance liquid chromatograph is an apparatus used to separate, identify, and quantify each component present in in a experimental matrix. Liquid chromatography is a fundamental separation technique in the life sciences and related fields of chemistry.
Waters HPLC system consisting of 1525 binary pumps, thermostat and 717+ autosampler connected to the Waters 2996 diode array and EMD 1000 single quadropole detector with the ESI probe. 2465 electrochemical detector is also included in different setups.

Capital equipment

Optical emission spectrophotometer with inductively coupled plasma (ICP-OES) “SpectroGenesis EOP II″.

Manufacturer: Spectro Analytical Instruments GmbH, Kleve, Germany.

Auxiliary equipment

2D Electrophoresis System “Multiphor II, EPS 3501 XL, SE 600 Ruby″ (Amersham BioSciences, UK)

Auxiliary equipment

PCR System “Applied Biosystems 2720″ (Applied Biosystems, USA)

Auxiliary equipment

Gel visualization system “GelDoc UVIDOC, UVIdoc D-55-LCD-20M-Auto″ (Uvitec, UK)

Auxiliary equipment

Laminar flow cabinet “AURA MINI″ (Laftech Bio-Cabinets, Australia)

Auxiliary equipment

BioRad Trans-Blot SD System (BioRad, USA)

Auxiliary equipment

Clark type oxygen electrode(Hansatech Instruments Ltd., UK)

Auxiliary equipment

Fully equipped biochemical lab

Biochemical lab contains: working surfaces, storage space, 3 centrifuge (Eppendorf, Germany), analytical scale (Sartorius, Germany), pH meter, shaker, microplate reader, mill.

Auxiliary equipment

Ultra-Low Temperature Freezer “Sanyo MDF-393″ (Sanyo, Japan)

Auxiliary equipment

Nitrogen Dry Box (glove-box for anoxic conditions) (Plas-Lab, USA)

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