University of Belgrade

Institute for
Multidisciplinary Research

Ivan Spasojević

Dr Ivan Spasojević

Research Professor

Department of Life Sciences (LS)

Phone: +381-11/2078-459


Curriculum vitae



2006: Ph.D. in Biophysics, University of Belgrade.


2002: B.Sc. in Molecular Biology and Physiology, Faculty of Biology, University of Belgrade.


Work Experience


2014-present: Head of the Department for Life Sciences


2012-present: Research Professor, Institute for Multidisciplinary Research, University of Belgrade.


2008-2009: Postdoctoral Fellow, Centre for Laser Microscopy, Institute for Physiology and Biochemistry, University of Belgrade.


2006-2012: Research Fellow (Research Assistant Professor), Institute for Multidisciplinary Research, University of Belgrade.


Scholarships and Awards


2008-2009: FP6 NEUROIMAGE Grant for Post Doc studies.


2003-2006: Grant of the Ministry of Science of the Republic of Serbia.


1998-2003: Grant of the Republic Foundation for Support of Young Talents.


Research Interests


• Redox processes.

• Free radicals and antioxidants.

• EPR spectroscopy.


Courses Taught


2010-2011: “The homeostasis of living systems”, undergraduate students seminar, Faculty of Chemistry, University of Belgrade.


2008-2009: “Biophysical Chemistry” for undergraduate students, Faculty of Physical Chemistry, University of Belgrade.


2006-2009: “The applications of EPR spectroscopy in Biophysical Chemistry”, undergraduate students seminar, Faculty of Physical Chemistry, University of Belgrade.


Other Professional Activities


• Membership: Serbian Biochemical Society (since 2011), Biophysical Society of Serbia (since 2007), Society of Physical Chemists of Serbia (since 2005).

• Chairman of the Publication Committee of Serbian Biochemical Society (since 2013).


Representative publications

In News:


Lab Times: Quick, Easy and Cheap Protein Visualization – ID=1114


SFRBMdot Article – ID=1018


What’s New in Shock? September 2014 – ID=1020


Fructose: Bad sugar\’s good deeds (2009) Functional Glycomics – ID=1019

Peer-reviewed international journals


Opačić M., Stević Z., Baščarević V., Živić M., Spasić M., Spasojević I. (2017) Can oxidation-reduction potential of cerebrospinal fluid be a monitoring biomarker in amyotrophic lateral sclerosis? Antioxidants and Redox Signaling doi: 10.1089/ars.2017.7433
(Endocrinology & Metabolism 13/138, IF = 6.337, M21a)
– ID=1434


Opačić M., Ristić A.J., Savić D., Šelih V.S., Živin M., Sokić D., Raičević S., Baščarević V., Spasojević I. (2017) Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. Metallomics 9: 141-148.
(Biochemistry & Molecular Biology 96/289, IF = 3.540, M22)
– ID=1382


Božić B., Korać J., Stanković D.M., Stanić M., Popović-Bijelić A., Bogdanović Pristov J., Spasojević I., Bajčetić M. (2017) Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine. Chemico-Biological Interactions 278: 129-134.
(Pharmacology & Pharmacy 27/92, IF = 3.143, M21)
– ID=1436


Martinov J., Krstić M., Spasić S., Miletić S., Stefanović-Kojić J., Nikolić-Kokić A., Blagojević D., Spasojević I., Spasić MB. (2017) Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. Food Research International 100: 132-136.
(Food Science & Technology 21/130, IF = 3.086, M21)
– ID=1435


Nehybová T., Šmarda J., Daniel L., Stiborek M., Kanický V., Spasojevič I., Preisler J., Damborský J., Beneš P. (2017) Wedelolactone acts as proteasome inhibitor in breast cancer cells. International Journal of Molecular Sciences 18(4): E729. – ID=1428


Milić Komić S., Bogdanović Pristov J., Popović-Bijelić A., Zakrzewska J., Stanić M., Kalauzi A., Spasojević I. (2016). Photo-redox reactions of indole and ferric iron in water. Applied Catalysis B: Environmental, 185, 174-180., (2014 Engineering, Environmental 1/47, IF=7.435, M21)
– ID=1174


Kezic A, Spasojevic I, Lezaic V, Bajcetic M. (2016) Mitochondria-targeted antioxidants: Future perspectives in kidney ischemia reperfusion injury. Oxidative Medicine and Cellular Longevity 2016: 2950503. (Cell Biology 53/187, IF = 4.492, M21)
– ID=1392


Spasojević I. (2016) What if cell culture media do not mimic in vivo redox settings? Redox Report 21(3): 127-129.
(Biochemistry & Molecular Biology 149/289, IF = 2.606, M22) – ID=1391


Marinković V., Ranković-Janevski M., Spasić S., Nikolić-Kokić A., Lugonja N., Djurović D., Miletić S., Vrvić M.M., Spasojević I. (2016) Antioxidative activity of colostrum and human milk: Effects of pasteurization and storage. Journal of Pediatric Gastroenterology & Nutrition 62(6): 901-906. (Pediatrics 27/120, IF = 2.400, M21) – ID=1390


Miljković D., Blaževski J., Petković F., Djedović N., Momčilović M., Stanisavljević S., Jevtić B., Mostarica Stojković M, Spasojević I. (2015) A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate. Journal of Immunology 194: 2493-2503. doi: 10.4049/jimmunol.1402302. – ID=1076


Milic S, BogdanovićPristov J., Mutavdzič D., Savic A., Spasic M, Spasojevic I. (2015) The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System, Environmental Science and Technology, 49(7): 4245-4254. – ID=1044


Ristić AJ., Savić D., Bogdanović Pristov J., Nestorov J., Baščarević V., Raičević S., Savić S., Spasojević I. (2015) Hippocampal antioxidative system in mesial temporal lobe epilepsy. Epilepsia In Press doi: 10.1111/epi.12981. – ID=1077


Nikolić-Kokić A., Oreščanin-Dušić Z., Spasojević I., Slavić M., Mijušković A., Paškulin R., Miljević Č., Spasić MB., Blagojević DP. (2015) Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. Journal of Ethnopharmacology 164: 64-70. doi: 10.1016/j.jep.2015.01.037. – ID=1078


Bogdanović Pristov J., Opačić M., Dimitrijević M., Babić N., Spasojević I. (2015) A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Analytical Biochemistry 480: 6-10. doi: 10.1016/j.ab.2015.04.006. – ID=1106


Bogosavljević V., Bajčetić M., Spasojević I. (2015) Comparative analysis of antioxidative systems in malignant and benign brain tumours. Redox Report 20: 69-74. doi: 10.1179/1351000214Y.0000000106. – ID=1107


Korenić A, Andjus P, Radenović L, Spasojević I. (2015) The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation. Neurosci Letters 595: 128-133. doi: 10.1016/j.neulet.2015.04.020. – ID=1108


Nikolić-Kokić A., Oreščanin-Dušić Z., Spasojević I., Blagojević D., Stević Z., Anđus P., Spasić M. (2015) The effects of wild-type and mutant SOD1 on smooth muscle contraction. Archives of Biological Sciences 67: 187-192 doi:10.2298/ABS141006023N – ID=1109


Spasojević I. (2015) What if cell culture media do not mimic in vivo redox settings? Redox Report doi: 10.1179/1351000215Y.0000000036 – ID=1110


Mojić M., Bogdanović Pristov J., Maksimović-Ivanić D., Jones D.R., Stanić M, Mijatović S, Spasojević I. (2014) Extracellular iron diminishes anticancer effects of vitamin C: an in vitro study. Scientific Reports 4: 5955. doi: 10.1038/srep05955. – ID=1016


Ristić AJ., Sokić D., Baščarević V., Spasić S., Vojvodić N., Savić S., Raičević S., Kovačević M., Savić D., Spasojević I. (2014) Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. Epilepsia 55: e34-e37 doi: 10.1111/epi.12593, Clinical Neurology 33/192, 3.909 – ID=901


Bajčetić M., Spasić S., Spasojević I. (2014) Redox therapy in neonatal sepsis: Reasons, targets, strategy, and agents. Shock 42: 179-184. Surgery 36/199, 2.612 – ID=903


Bogosavljević V., Bajčetić M., Spasojević I. (2014) Comparative analysis of antioxidative systems in malignant and benign brain tumours. Redox Report 20: 69-74. doi: 10.1179/1351000214Y.0000000106 – ID=1017 – ID=1017


Mijušković A., Oreščanin-Dušić Z., Nikolić-Kokić A., Slavić M., Spasić MB., Spasojević I., Blagojević D. (2014) Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. Pharmacological Reports 66: 373-379. doi: 10.1016/j.pharep.2013.12.012 – ID=902


Spasojević I. (2014) Integrative concept of homeostasis: translating physiology into medicine. F1000Research 3: 299. – ID=1429


Miljković D., Spasojević I. (2013) Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities. Antioxidants and Redox Signaling 19: 2286-2334. doi:10.1089/ars.2012.5068. Biochemistry & Molecular Biology 32/290 Endocrinology & Metabolism 10/122 7.189
– ID=876


Bajić A., Spasić M., Andjus P.R., Savić D., Parabucki A., Nikolić-Kokić A., Spasojević I. (2013) Fluctuating vs. continuous exposure to H2O2: The effects on mitochondrial membrane potential, intracellular calcium, and NF-kappaB in astroglia. PLoS One In Press. Multidisciplinary Sciences 7/56, 3.730 – ID=877


Sonmez M., Ince H.Y., Yalcin O., Ajdžanović V., Spasojević I., Meiselman H.J., Baskurt O.K. (2013) The effect of alcohols on red blood cell mechanical properties and membrane fluidity depends on their molecular size. PLoS One In Press. Multidisciplinary Sciences 7/56, 3.730 – ID=878


Bogdanović Pristov J., Veljović Jovanović S., Mitrović A., Spasojević I. (2013) UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid. Physiolgia Plantarum, doi: 10.1111/ppl.12001. Plant Sciences 24/197 3.656 – ID=722


Ajdžanović V., Mojić M., Maksimović-Ivanić D., Bulatović M., Mijatović S., Milošević V., Spasojević, I. (2013) Membrane fluidity, invasiveness and dynamic phenotype of metastatic prostate cancer cells after treatment with soy isoflavones. Journal of Membrane Biology 246: 307-314. – ID=986


Nikolić-Kokić A., Oreščanin-Dušić Z., Slavić M., Spasojević I., Stević Z., Spasić M., Blagojević D. (2013) The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions. Journal of Medical Biochemistry 32: 375-379. – ID=987


Bogdanović Pristov J., Spasić M., Spasojević I. (2013) Converting low dose radiation to redox signaling. Plant Signaling and Behavior 8: e23151. – ID=988


Bajčetić M., Otašević B., Prekajski N.B., Spasić S., Spasojević I. (2014) Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. Annals of Clinical Biochemistry 51: 550-556. doi: 10.1177/0004563213503317. – ID=989


Žižić M., Živić M., Spasojević I., Bogdanović Pristov J., Stanić M., Cvetić-Antić T., Zakrzewska J. (2013) The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. Research in Microbiology 164: 61-69 – ID=721


Lugonja N., Spasić S.D., Laugier O., Nikolić-Kokić A., Spasojević I, Orepčanin-Dušić Z., Vrvić M. (2013) Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. Nutrition, doi: 10.1016/j.nut.2012.07.018 – ID=723


Spasojević I., Obradović B., Spasić S. (2012) Bench-to-bedside review: Neonatal sepsis – redox processes in pathogenesis. Critical Care 16: 221. Critical Care Medicine 5/27 4.718 – ID=716


Spasojević I., Bogdanović Pristov J., Vujisić Lj., Spasić M. (2012) The reaction of methionine with hydroxyl radical: Reactive intermediates and methanethiol production. Amino Acids 42: 2439-2445. Biochemistry & Molecular Biology 86/290, 3.914 – ID=62


Pristov J.B., Maglić D., Opačić M., Mandić V., Miković Ž., Spasić M., Spasojević I. (2012) Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin. Thrombosis Research 130: 826-829. Peripheral Vascular Disease 18/68 3.133 – ID=718


Ajdžanović V., Spasojević I., Pantelić J., Šošić-Jurjević B., Filipović B., Milošević V., Severs W. (2012) Vitex agnus-castus l. essential oil increases human erythrocyte membrane fluidity. Journal of Medical Biochemistry 31: 222-227. – ID=985


Ignjatović A., Stević Z., Lavrnić D., Nikolić-Kokić A., Blagojević D., Spasić M., Spasojević I. (2012) Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. Amyotrophic Lateral Sclerosis 13: 357-362 – ID=715


Ajdžanović V.Z., Milošević V.Lj., Spasojević I.B. (2012) Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial. General Physiology and Biophysics 31: 367-374 – ID=717


Pejin B., Kien-Thai Y., Bogdanovic-Pristov J., Pejin I., Spasojević I. (2012) In vitro investigation of the antihypertensive effect of the moss Rhodobryum ontariense (Kindb.). Digest Journal of Nanomaterials and Biostructures 7: 353-359 – ID=719


Spasojević I., Jones D.R., Andrades M.E. (2012) Hydrogen peroxide in adaptation. Oxidative Medicine and Cellular Longevity, Article ID 596019, 4 pages – ID=720


Spasojević I. (2011) Free radicals and antioxidants at a glance using EPR spectroscopy. Critical Reviews in Clinical Laboratory Sciences 48: 114-142. Medical Laboratory Technology 2/32 5.230 – ID=711


Аndrades М.Е., Morina А., Spasić S., Spasojević I. (2011) Bench-to-bedside review: Sepsis – from the redox point of view. Critical Care 15: 230. Critical Care Medicine 5/26, 4.607 – ID=712


Bogdanović Pristov J., Mitrović A., Spasojević I. (2011) A comparative study of antioxidative activities of cell-wall polysaccharides. Carbohydrate Research 346: 2255-2259. Chemistry, Applied 18/71 2.332 – ID=59


Bajić A., Zakrzewska J., Godjevac D., Andjus P., Jones D.R., Spasić M., Spasojević I. (2011) Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. Carbohydrate Research 346: 416-420. Chemistry, Applied 18/71 2.332 – ID=710


Ajdžanović V., Spasojević I., Šošić-Jurjević B., Filipović B., Trifunović S., Sekulić M., Milošević V. (2011) The negative effect of soy extract on erythrocyte membrane fluidity: an electron paramagnetic resonance study. Journal of Membrane Biology 239: 131-135 – ID=713


Spasojević I., Mojović M., Ignjatović A., Bačić G. (2011) The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds. Journal of the Serbian Chemical Society 76: 647-677 – ID=714


Spasojević I., Mojović M., Stević Z., Spasić S.D., Jones D.R., Morina A., Spasić M.B. (2010) Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. Redox Report 15: 29-39 – ID=707


Spasojević I., Stević Z., Nikolić-Kokić A., Jones D.R., Blagojević D., Spasić M.B. (2010) Different roles of radical scavengers – ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. Redox Report 15: 81-86 – ID=708


Ajdžanović V., Spasojević I., Filipović B., Šošić-Jurjević B., Sekulić M., Milošević V. (2010) Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. Canadian Journal of Physiology and Pharmacology 88: 497-500 – ID=709


Spasojević I., Bogdanović Pristov J. (2010) The potential physiological implications of polygalacturonic acid-mediated production of superoxide. Plant Signaling & Behavior 5(12): 1-5 – ID=58


Bogdanović J., Spasojević I., Miković Ž., Mandić V., Cerović N., Spasić M. (2009) Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. Oxidative Medicine and Cellular Longevity 2: 14-18 – ID=55


Spasojević I., Mojović M., Blagojević D., Spasić S., Jones D., Nikolić- Kokić A., Spasić M. (2009) Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. Carbohydrate Research 344: 80-84 – ID=700


Spasojević I., Bajić A., Jovanović K., Spasić M., Andjus P. (2009) Protective role of fructose in the metabolism of astroglial C6 cells exposed to hydrogen peroxide. Carbohydrate Research 344: 1676-1681 – ID=701


Živković J., Zeković Z., Mujić I., Gođevac D., Mojović M., Mujić A., Spasojević I. (2009) EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of Castanea sativa. Food Biophysics 4: 126-133 – ID=702


Živković J., Zeković Z., Mujić I., Tumbas V., Cvetković D., Spasojević I. (2009) Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts. Food Technology and Biotechnology 47: 421-427 – ID=703


Kojić D., Spasojević I., Mojović M., Blagojević D., Worland M.R., Grubor-Lajšić G., Spasić M.B. (2009) Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). European Journal of Entomology 106: 451-454 – ID=704


Spasojević I., Jelić S., Zakrzewska J., Bačić G. (2009) Decreased oxygen transfer capacity of erythrocytes as a cause of 5-fluorouracil related ischemia. Molecules 14: 53-67 – ID=705


Oreščanin-Dušić Z., Milovanović S., Nikolić-Kokić A., Radojčić R., Spasojević I., Spasić M. (2009) Diethyldithiocarbamate potentiates the effects of protamin sulphate in the isolated rat uterus. Redox Report 14: 48-54 – ID=706


Gođevac D., Vujisić L., Mojović M., Ignjatović A., Spasojević I., Vajs V. (2008) Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity. Food Chemistry 107: 1692-1700. Food Science & Technology 9/107 Nutrition & Dietetics 17/59 2.696 – ID=698


Bogdanović J., Mojović M., Milosavić N., Mitrović A., Vučinić Ž., Spasojević I. (2008) Role of fructose in the adaptation of plants to cold-induced oxidative stress. European Biophysics Journal with Biophysics Letters 37: 1241-1246 – ID=51


Bačić G., Spasojević I., Šećerov B., Mojović M. (2008) Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. Spectrochimica Acta A 69: 1354-1366 – ID=699


Mojović M., Spasojević I., Bačić G. (2005) Detection of hydrogen atom adduct of spin-trap DEPMPO. Relevance for studies of biological systems. Journal of Chemical Information and Modeling 25: 1716-1718. Chemistry, Multidisciplinary 19/124, Computer Science, Information Systems 6/83, Computer Science, Interdisciplinary Applications 6/83, 2.923 – ID=696


Spasojević I., Maksimović V., Zakrzewska J., Bačić G. (2005) Effects of 5-Fluorouracil on Erythrocytes in Relation to Its Cardiotoxicity: Membrane Structure and Functioning. Journal of Chemical Information and Modeling 45(6): 1680-1685. Chemistry, Multidisciplinary 19/124, Computer Science, Information Systems 6/83, Computer Science, Interdisciplinary Applications 6/83, 2.923 – ID=473


Spasojević I., Zakrzewska J., Bačić G. (2005) 31P NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil: Cardiotoxicity related changes in ATP, 2,3-BPG and O2 metabolism. Annals of the New York Academy of Sciences 1048: 311-320. Multidisciplinary Sciences 5/48 1.971 – ID=695


Spasojević I., Maksimović V., Bačić G. (2005) 5-Fluorouracil effects on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning. Journal of Chemical Information and Modeling 45: 1680-1685 – ID=694


Mojović M., Spasojević I., Vuletić M., Vučinić Ž., Bačić G. (2005) EPR spin-probe and spin-trap study of free radicals produced by plant plasma membranes. Journal of the Serbian Chemical Society 70: 177-186 – ID=697


Current projects

Project ID
Project Title
Financial Source
My Status
Cost Action CA15133 The Biogenesis of Iron-sulfur Proteins: from Cellular Biology to Molecular Aspects (FeSBioNet) COST (European Cooperation in Science and Technology) Member
SPS G5320 Radiation Hormesis for Higher Microalgae Biofuels Yield NATO Science for Peace and Security Programme Project Leader
OI173014 Molecular mechanisms of redox signaling in homeostasis, adaptation and pathology Ministry of Education, Science and Technological Development of the Republic of Serbia Member
OI173017 Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering Ministry of Education, Science and Technological Development of the Republic of Serbia Member